Chiral HPLC method development PZQAmine at Syncom B.V.

There is good news regarding the separation of the racemic praziquanamine with chiral HPLC.

• Syncom B.V., The Netherlands, a CRO specialized in all aspects of organic synthesis, with a 20-year track record in resolution of chiral compounds, screened five different chiral HPLC columns, varied the eluent and used UV detection (220 and 260 nm) and a PDR-Chiral Advanced Laser Polarimeter.

 

• The S(+)- and R(-)-enantiomers of praziquanamine could be separated well with good baseline resolution using the Chiralcel OJ-H, as well as the Chiralpak IA, and the Chiralpak AS-H column with heptane/EtOH/Et2NH (60/40/0.2) as eluent (0.5 ml/min flow rate).

• The Chiralpak OD-H or the Chiralpak IB column was not suitable for the separation of these enantiomers, as was also noticed in Eur.J.Org.Chem. 2008, 895.

 

The diastereomeric salt resolution screening of praziquanamine with some chiral acids is in progress.

 

Jean-Paul.

j.p.g.seerden@syncom.nl

http://www.syncom.nl

Note - the raw data from this trial (HPLC traces for all the different columns) may be downloaded with this link. (large PDF file)