Chromatography of PZQ

Published by RyanPakula on 20 October 2009 - 2:43am

Methods for the purification of PZQ can be collated here.
 
Literature
1. This paper describes the purification of enantiopure PZQ using "chloroform/methanol 0–0.3% MeOH as a solvent system."
2. This paper uses 1:1 EtOAc/petroleum ether ramping to pure EtOAc.
3. This paper uses "thick layer preparative chromatography [prep TLC] benzene/ethyl acetate 1/1, silica gel."
 

Comments

MatTodd's picture

There are many ways to separate PZQ enantiomers by analytical HPLC. A sample trace is posted as an attachment to this comment. Conditions + retention times: Chiralcel OD, Hex/IPA/TEA 60:40:0.1, flow rate 0.7 mL per minute. Retention times 11.6 and 13.6 minutes.